VWR Healthcare Clinical Catalog 2012-2014 - page 350

E. cloni® Competent Cells, Lucigen
Developed for the toughest cloning and protein expression jobs, E. cloni competent cells are optimized to give high yields of recombinant
plasmid, cosmid, or BAC DNAs. These cells share the most useful genetic elements of standard cloning and expression strains like DH5[alpha]®,
DH10B™, or BL21 (DE3), and can directly replace them in cloning and expression protocols. However, E. cloni cells feature technology that increases transformation
efficiency and reliability, while decreasing costs.
10G SUPREME electrocompetent cells are designed for the most demanding cloning projects or for creating large, complex libraries. 10G ELITE electrocompetent cells
are meant to be used with difficult-to-clone DNA or library construction. 10G CLASSIC electrocompetent cells are ideal for general high efficiency cloning. 10G and
10GF' chemically competent cells are meant for rountine cloning and subcloning. All of the 10G cells are phage resistant and can be used to clone cosmids, BAC, and
methylated DNA. They can also be used in blue/white screening protocols without IPTG induction.
10GF' ELITE electrocompetent cells are designed for ssDNA production or general high efficiency cloning of methylated DNA. They can also be used in blue/white
screening protocols; however, unlike the 10G cells, the 10GF' cells require IPTG induction. These cells are not appropriate for use in BAC or cosmid cloning.
TG1 electrocompetent cells are designed for phage display protein expression, and are also suitable for M13 phage work, general cloning, and blue/white screening.
The 5-Alpha chemically competent cells are versatile enough for use in a wide variety of applications, such as routine cloning, subcloning, and plasmid isolation with
or without blue/white screening.
EXPRESS BL21 (DE3) electrocompetent cells are designed to enable researchers to clone and express proteins in the same cell. They can be used with cosmid, BAC,
and methylated DNA to construct complex expression libraries. EXPRESS BL21 (DE3) chemically competent cells are meant to be used in routine protein expression
protocols. These can also be used with cosmid, BAC, and methylated DNA. Both EXPRESS electrocompetent and chemically competent cells are also available with a
chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of
lysozyme, while the pLysE cells produce a larger amount of lysozyme. The EXPRESS line of cells is not appropriate for use with blue/white screening protocols.
The OverExpress™ line of cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms. They contain genetic mutations phenotypi-
cally selected for conferring tolerance to toxic proteins. The C41(DE3) strain was derived from BL21(DE3) and has at least one uncharacterized mutation to prevent cell
death associated with expression of many recombinant toxic proteins. The C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein
to enable the C43(DE3) cells to express a different set of toxic proteins. As in standard BL21(DE3) strains, pLysS cells produce a small amount of lysozyme. OverExpress
cells are also deficient in the Ion and ompT proteases. OverExpress strains can be differentiated from each other and from BL21(DE3) by transformation with the plas-
mid verification vector pAVD10. This vector is lethal to BL21(DE3) and to induced C41(DE3), but tolerated by C43(DE3), regardless of induction. The vector is supplied
with all OverExpress cells.
Ordering Information: SOLO packaging contains enough cells for one reaction/tube. DUO packaging contains enough cells for two reactions/tube. SixPack packaging
contains enough cells for six reactions/tube. All cells come with pUC control DNA and either recovery medium or expression recovery medium to further boost recom-
binant yields after transformation. ComboPacks contain all of the EXPRESS electrocompetent (89047-688), EXPRESS chemically competent (89027-154), OverExpress
electrocompetent (89027-132), or OverExpress chemically competent (89027-150) strains in one kit, along with the expression recovery medium and pUC control DNA.
OverExpress ComboPacks also come with pAVD10 verification plasmid.
Description Size Packaging Supplier No. Cat. No. Price
10G Chemically Competent Cells (≥1 x 108 cfu/μg DNA) 12 Reactions SOLOS 60106-1 89005-078 Ea./ 111.24
10G Chemically Competent Cells (≥1 x 108 cfu/μg DNA) 24 Reactions SOLOS 60106-2 89005-080 Ea./ 195.70
10G Chemically Competent Cells (≥1 x 108 cfu/μg DNA) 48 Reactions SOLOS 60106-3 89005-082 Ea./ 326.51
10G Chemically Competent Cells (≥1 x 108 cfu/μg DNA) 12 Reactions DUOS 60107-1 89002-674 Ea./ 98.88
10G Chemically Competent Cells (≥1 x 108 cfu/μg DNA) 24 Reactions DUOS 60107-2 89002-676 Ea./ 170.98
10G Chemically Competent Cells (≥1 x 108 cfu/μg DNA) 48 Reactions DUOS 60107-3 89002-678 Ea./ 283.25
10G Chemically Competent Cells (≥1 x 108 cfu/μg DNA) 96 Reactions DUOS 60107-4 89002-680 Ea./ 504.70
10G CLASSIC Electrocompetent Cells (≥5 x 109 cfu/μg DNA) 24 Reactions SixPacks 60117-1 89002-684 Ea./ 157.59
10G CLASSIC Electrocompetent Cells (≥5 x 109 cfu/μg DNA) 48 Reactions SixPacks 60117-2 89002-686 Ea./ 283.25
10G ELITE Electrocompetent Cells (≥2 x 1010 cfu/μg DNA) 12 Reactions SOLOS 60051-1 89005-074 Ea./ 159.65
10G ELITE Electrocompetent Cells (≥2 x 1010 cfu/μg DNA) 24 Reactions SOLOS 60051-2 89005-076 Ea./ 277.07
10G ELITE Electrocompetent Cells (≥2 x 1010 cfu/μg DNA) 12 Reactions DUOS 60052-1 89002-658 Ea./ 139.05
10G ELITE Electrocompetent Cells (≥2 x 1010 cfu/μg DNA) 24 Reactions DUOS 60052-2 89002-660 Ea./ 241.02
10G ELITE Electrocompetent Cells (≥2 x 1010 cfu/μg DNA) 24 Reactions SixPacks 60052-3 89002-662 Ea./ 184.37
10G ELITE Electrocompetent Cells (≥2 x 1010 cfu/μg DNA) 48 Reactions SixPacks 60052-4 89002-664 Ea./ 306.94
10G SUPREME Electrocompetent Cells (≥4 x 1010 cfu/μg DNA) 12 Reactions DUOS 60080-1 89002-670 Ea./ 221.45
10G SUPREME Electrocompetent Cells (≥4 x 1010 cfu/μg DNA) 24 Reactions DUOS 60080-2 89002-672 Ea./ 407.88
10G SUPREME Electrocompetent Cells (≥4 x 1010 cfu/μg DNA) 12 Reactions SOLOS 60081-1 95024-012 Ea./ 252.35
10G SUPREME Electrocompetent Cells (≥4 x 1010 cfu/μg DNA) 24 Reactions SOLOS 60081-2 95024-014 Ea./ 449.08
10GF' Chemically Competent Cells (≥5 x 108 cfu/μg DNA) 12 Reactions DUOS 60062-1 95024-016 Ea./ 98.88
10GF' Chemically Competent Cells (≥5 x 108 cfu/μg DNA) 24 Reactions DUOS 60062-2 95024-018 Ea./ 170.98
10GF' ELITE Electrocompetent Cells (≥2 x 1010 cfu/μg DNA) 12 Reactions DUOS 60061-1 89002-666 Ea./ 139.05
10GF' ELITE Electrocompetent Cells (≥2 x 1010 cfu/μg DNA) 24 Reactions DUOS 60061-2 89002-668 Ea./ 241.02
5-Alpha Chemically Competent Cells (≥1 x 108 cfu/μg DNA) 12 Reactions DUOS 60602-1 95040-454 Ea./ 98.88
5-Alpha Chemically Competent Cells (≥1 x 108 cfu/μg DNA) 24 Reactions DUOS 60602-2 95040-456 Ea./ 170.98
96-Well 10G Chemically Competent Cells (≥1 x 108 cfu/μg DNA) 1 Plate 60096-1 95040-458 Ea./ 362.56
96-Well 10G Chemically Competent Cells (≥1 x 108 cfu/μg DNA) 4 Plates 60096-4 95040-460 Ea./ 1275.14
EXPRESS BL21 (DE3) Chemically Competent Cells (≥1 x 107 cfu/μg DNA) 12 Reactions DUOS 60401-1 89005-032 Ea./ 59.74
EXPRESS BL21 (DE3) Chemically Competent Cells (≥1 x 107 cfu/μg DNA) 24 Reactions DUOS 60401-2 89005-034 Ea./ 85.49
EXPRESS BL21 (DE3) Chemically Competent Cells (≥1 x 107 cfu/μg DNA) 48 Reactions DUOS 60401-3 89005-036 Ea./ 124.63
EXPRESS BL21 (DE3) Electrocompetent Cells (≥5 x 109 cfu/μg DNA) 12 Reactions DUOS 60300-1 89005-020 Ea./ 96.60
EXPRESS BL21 (DE3) Electrocompetent Cells (≥5 x 109 cfu/μg DNA) 24 Reactions DUOS 60300-2 89005-022 Ea./ 172.20
EXPRESS BL21 (DE3) pLysE Chemically Competent Cells (≥1 x 107 cfu/μg DNA) 12 Reactions DUOS 60425-1 89005-044 Ea./ 59.74
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Molecular Diagnostics
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