VWR Healthcare Clinical Catalog 2012-2014 - page 346

PerfeCTa® qPCR SuperMixes, Quanta Biosciences
Unique buffer and stabilizers have been specifically optimized to deliver maximum PCR
efficiency, sensitivity, and a robust fluorescent signal with TaqMan® or TaqMan® MGB probe
chemistry. The enhanced specificity of this supermix suppresses cross-reactivity between
homologous sequences, improving detection and discrimination in SNP applications. A key
component of this supermix is AccuStart™ Taq DNA polymerase with monoclonal antibodies
that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step.
Upon heat activation (two minutes at 95°C), the antibodies denature irreversibly, releasing
fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer
extension with the convenience of room temperature reaction assembly.
Supermixes are available and optimized for all real-time PCR instrument platforms including
those requiring normalization with ROX™ dyes. Supermixes are also available with uracil-
N-glycosylase (UNG) to prevent amplification of carry-over contamination from previous
dU-containing PCRs.
Reactions/Kit Supplier No. Cat. No. Price
SuperMix
100 95050-100 101414-118 Ea./ 177.00
500 95050-500 101414-120 Ea./ 869.00
2000 95050-02K 101414-114 Ea./ 3274.00
SuperMix with Low ROX ™
100 95052-100 101414-134 Ea./ 177.00
500 95052-500 101414-136 Ea./ 869.00
2000 95052-02K 101414-130 Ea./ 3274.00
SuperMix with ROX™
100 95051-100 101414-126 Ea./ 177.00
500 95051-500 101414-128 Ea./ 869.00
2000 95051-02K 101414-122 Ea./ 3274.00
SuperMix with UNG
100 95064-100 101414-210 Ea./ 186.00
500 95064-500 101414-212 Ea./ 913.00
2000 95064-02K 101414-206 Ea./ 3438.00
SuperMix with UNG and Low ROX™
100 95066-100 101414-226 Ea./ 186.00
500 95066-500 101414-228 Ea./ 913.00
2000 95066-02K 101414-222 Ea./ 3438.00
SuperMix with UNG and ROX ™
100 95065-100 101414-218 Ea./ 186.00
500 95065-500 101414-220 Ea./ 913.00
2000 95065-02K 101414-214 Ea./ 3438.00
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Molecular Diagnostics
PCR Kits