qScript
™
One-Step Fast and Fast MGB qRT-PCR Kits, Quanta BioSciences
Kits offer a convenient and highly sensitive solution for real-time reverse transcriptase PCR (qRT-PCR) of RNA templates using hybridiza-
tion probe detection chemistries such as TaqMan
®
5'-hydrolysis probes or molecular beacons on PCR instrument platforms.
For Minor Groove Binder (MGB) modified probes, qScript
™
One-Step fast MGB qRT-PCR kits are recommended. These kits offer a con-
venient and highly sensitive solution for qRT-PCR of RNA templates using TaqMan-MGB 5'-hydrolysis probe detection exclusively on
qRT-PCR instrument platforms.
The system allows cDNA synthesis and PCR amplification to be carried out in the same tube without opening between procedures. It is ideal for highly sensitive quan-
tification of RNA viruses or low abundance RNA targets, as wells as high throughput gene-expression studies. It has also been optimized to deliver maximum RT-PCR
efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times.
One-step fast master mixes are provided as a 4x concentrated solution to allow addition of higher amounts of RNA template and improved detection sensitivity with
low concentration samples. The unique formulation maximizes the activities of both reverse transcriptase and Taq DNA polymerase while minimizing the potential for
primer-dimer and other non-specific PCR artifacts. This enables unbiased co-amplification of low copy transcripts in the presence of higher copy reference genes in
duplexed qRT-PCR applications.
Highly-specific amplification is crucial to successful qRT-PCR, as non-specific product(s) can compete for amplification of the target sequence and impair PCR ef-
ficiency. A key component of these kits is AccuStart
™
Taq DNA polymerase with monoclonal antibodies that bind to the polymerase and keep it inactive during reaction
assembly and the 50°C reverse transcription step. A brief 30-second heat activation step at 95°C irreversibly denatures the antibodies, releasing fully active, unmodi-
fied Taq DNA polymerase. Rapid recovery of fully active, unmodified Taq DNA polymerase is critical for efficient extension kinetics. Therefore, these kits afford greater
reagent economy and laboratory throughput on conventional or rapid ramp rate qPCR systems.
Kits are available for all real-time PCR instrument platforms including those requiring normalization with ROX
™
reference dye. Platforms not requiring passive refer-
ence dye include Roche LightCycler
®
480, Bio-Rad
®
iQ
™
, MyiQ
™
, iQ
™
5, Opticon
®
, Chromo4
™
, Corbett Rotor-Gene
®
, Eppendorf
®
Mastercycler
®
, and Cepheid
®
SmartCy-
cler
®
. Kits with Low ROX
™
reference dye are for use with platforms including AB 7500 and Stratagene
®
Mx
™
. Kits with ROX
™
reference dye are for use with platforms
including AB 7000, 7300, 7700, 7900, and StepOne
™
.
Ordering Information:
If not using Minor Groove Binder (MGB) modified probes exclusively, qScript
™
One-Step Fast qRT-PCR kits are recommended.
Reactions/Kit
Supplier No.
Cat. No.
Price
One-Step Fast qRT-PCR Kits
100
95079-100
Ea./
134.00
500
95079-500
Ea./
606.00
One-Step Fast qRT-PCR Kits, Low ROX
™
100
95081-100
Ea./
134.00
500
95081-500
Ea./
606.00
One-Step Fast qRT-PCR Kits, ROX
™
100
95080-100
Ea./
134.00
500
95080-500
Ea./
606.00
One-Step Fast MGB qRT-PCR Kits
100
95082-100
Ea./
134.00
500
95082-500
Ea./
606.00
One-Step Fast MGB qRT-PCR Kits, Low ROX
™
100
95084-100
Ea./
134.00
500
95084-500
Ea./
606.00
One-Step Fast MGB qRT-PCR Kits, ROX
™
100
95083-100
Ea./
134.00
500
95083-500
Ea./
606.00
Citrate Buffer, 10X, for Heat-Induced Epitope Recovery, Diagnostic Biosystems
This buffer is a concentrated antigen unmasking solution and is free from heavy metals. Fixative used on tissue sections to conserve the tissue from
autolysis, preserve tissue structures, and immobilize antigens alters the tissue. Antigens undergo chemical alteration of their primary, secondary and
tertiary structures. Because of changes in the protein-containing epitopes or in neighboring proteins, antigenic sites may be masked. Use of a heavy
metal solution in a microwave can regain the masked antigens significantly. However, heavy metals increase the risk of exposure of lab personnel
to lead. Use of this antigen unmasker can eliminate the risk of heavy metal exposure to lab personnel and also resolve heavy metals handling and
disposal problems.
Description
Supplier No.
Cat. No.
Price
Citrate Buffer, pH 6.0
K 035
Ea./
73.00
Citrate Buffer, pH 7.0
K 036
Ea./
73.00
Citrate Buffer, pH 8.0
K 037
Ea./
73.00
Citrate Buffer, pH 9.0
K 038
Ea./
73.00
1.800.932.5000
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Molecular Diagnostics
PCR Kits
