VWR Healthcare Clinical Catalog 2012-2014 - page 347

PerfeCTa
®
SYBR
®
Green FastMix
®
Reaction Mixes, Quanta Biosciences
This unique combination of proprietary buffer, stabilizers, and AccuFast
Taq DNA polymerase delivers maximum PCR efficiency, sen-
sitivity, and specificity, along with a robust fluorescent signal using fast, or conventional, cycling protocols with SYBR
®
Green qPCR.
Highly specific amplification is crucial to successful qPCR with SYBR
®
Green I dye technology because this dye binds to and detects
any dsDNA generated during amplification.
AccuFast
Taq DNA polymerase is a key component of this reaction mix. This hot-start Taq contains a unique mixture of monoclonal antibodies that bind to the
polymerase and keep it inactive prior to the initial PCR denaturation step (>48 hours at room temperature). Similar to our AccuStart
Taq DNA polymerase, these
antibodies are irreversibly inactivated during the initial PCR denaturation step. However, unlike other antibody hot-start polymerases, activation of AccuFast
Taq is
instantaneous at 95°C. Rapid recovery of fully active, unmodified Taq DNA polymerase is critical for efficient extension kinetics. Replication of fragments up to 200bp
is complete in less than 20 seconds at 60°C.
Kits are available for all real-time PCR instrument platforms including those requiring normalization with ROX
reference dye. Platforms not requiring passive refer-
ence dye include Roche LightCycler
®
480, Bio-Rad
®
iQ
, MyiQ
, iQ
5, Opticon
®
, and Chromo4
, Corbett Rotor-Gene
®
, Eppendorf
®
Mastercycler
®
, and Cepheid
®
Smart-
Cycler
®
. Kits with Low ROX
reference dye are for use with platforms including AB 7500 and Stratagene
®
Mx
. Kits with ROX
reference dye are for use with platforms
including AB 7000, 7300, 7700, 7900, and StepOne
.
Reactions/Kit
Supplier No.
Cat. No.
Price
Green FastMix
®
250
95072-250
Ea./
197.00
1250
95072-012
Ea./
890.00
5000
95072-05K
Ea./
3315.00
Green FastMix
®
for iQ
250
95071-250
Ea./
197.00
1250
95071-012
Ea./
890.00
5000
95071-05K
Ea./
3315.00
Green FastMix
®
, Low ROX
250
95074-250
Ea./
197.00
1250
95074-012
Ea./
890.00
5000
95074-05K
Ea./
3315.00
Green FastMix
®
, ROX
250
95073-250
Ea./
197.00
1250
95073-012
Ea./
890.00
5000
95073-05K
Ea./
3315.00
SampleCollection/Phlebotomy
AlcoholWipes
Biohazard Specimen Bags
Biopsy Punches
Bandages,Gauze,Wraps
Basins
Blood Collection Tubes
BloodDraw Chairs/Lounges
Blood TubeHolders
Caps/Closures
Capillary Tubes
Centrifuge Tubes
Collection Swabs
DOT/Temperature Compliant
Shippers
Feces Collection
Gel Packs
Gloves
HandHygiene
Histobrush, Cervical Brushes
and Scrapers
Lancets
Microtainers/MAP tubes
Nasal Swabs
Needles/Push ButtonNeedles
Phlebotomy Carts/Trays
Sharps Containers
Stool Specimen Collection
Swabs
Swabs,DNA-Free
Syringes
Transport Bags
Tubes and Containers
Urinalysis Collection
Urine Sample Containers,
24Hrw/wo Preservatives
Histology/Cytology
Alcohol
BaseMolds,Disposable and
Metal
Blades and Scissors
Bluing Reagent
Cassettes
Control Slides
Coverslips
Cytology Funnels,
Clips, and Slides
Decalcifiers
Forceps
Formalin
FormalinAbsorbant Pads
FormalinNeutralizer
H&E Stains
Hoods
IHCKits/Antibodies
Knives
Marking Pens
Megacassettes
Microwaves
Microtome Blades
Microtomes and Cryostats
Paraffin
ParaffinDispensers
Paraguard
Prefilled Formalin Containers
Scalpels and Blades
Slide and Cassette Storage
SlideWarmers/Dryers
Slides, Plain and Charged
Specimen Containers
Staining Racks
Stains
Storage Bags
Tissue FreezingMedium
Tissue Processors
Water Baths
Work Stations
Wright andWright
Giemsa Stains
Xylene
Xylene Substitutes
Hematology
Bleeding TimeDevices
Blood Bank Saline
Blood Rotators
Calibrators and Controls
Coagulation Controls
Coverslips
Differential Counters
ESR Testing
Hemacytometers, Standard
andDisposable
Microhematocrit Centrifuges
and Tubes
Microscope Slides
Microscopes
RBCDilution Cups
Sickle Cell Testing
Stains
Stainers
Statspin
Tourniquets
Tube Rotators
Tubes
Wipes
BloodBank
AABB Thermometers
Applicator Sticks
Bench Cover/ Roll
Blood Bank Saline
Copper Sulfate Solution
CellWashers
Cryo, FFP Bath
Disposable Culture Tubes
Freezers
Heating Blocks
Percoll/Ficol
Phlebotomy Chairs
Racks
Refrigerators
Robotic Tips
Segment Processing
Storage and Transport
Containers
TemperatureMonitoring
Viewers
Microbiology
Anaerobic Systems
Autoclave Bags, Controls,
and Tape
Chromagenic Plates
Contact Plates
ControlOrganisms
Disinfectants
Flasks
Gram Stains
Incinerators
Incubators, CO
2
Incubators,General Purpose
InhibitionDisks
Innoculating Loops
Media
Microincinerators
Parasitology Collection
PetriDishes
PlatedMedia
QCOrganisms
Slides
Stains
StrepGrouping Tests
Susceptibility TestingKits
Swabs, Collection and Transport
Swabs, Culture
TB Syringes
Viral Transport Systems
Wipers
MolecularDiagnostics
Aerosol Tips
DNA/RNA Purification Products
Enzymes
Microarray Slides
Molecular Biology Reagents
Paxgene Tubes
PCR Plates
PCR Tubes/Caps and Racks
Plate Readers andWashers
Sealing Film
Taq
Thermocyclers
Tissue CultureMedia
TissueGrinders
General Laboratory
AbsorbantUnderpads
Alarms
Autoclave Bags
Bleach Tablets
Bottle TopDispensers
Carboys
Click Seal Cups
Conical Tubes (15/50mL)
Cryogenic Tubes
Disinfectants/Detergents
Drapes
FiltrationUnits
Flasks and Cylinders
Funnels
Gauze Pads/Sponges
Glassware
Hemostats
Labels/Tape
Lancets
Lock Boxes
Parafilm
pH Buffers, Papers
Pipet Tips, Filtered
and Standard
Pipet Tips, Robotic
Pipets, Serological
Pipets, Transfer
Plasticware
Racks and Trays
Replacement Bulbs
Sample Cups, Sterile
andNon-Sterile
Serum Filters
Sterilization Pouches
SurgeryKits
Surgical Supplies, Scissors,
Scalpels, and Forceps
Syringe Filters
Syringes/Needles
Thermometers
Timers
Transport Tubes
Tubing
Wipers
ClinicalChemistry
BenchtopAnalyzer
Chemistry Controls
Centrifuges
Chromatography Fittings,
Syringes, andVials
Clinical Chemistry Controls
Cuvettes and Sampling Cups
DistilledWater
DrugsOfAbuse TestingKits
Freezers
GenericAnalyzer
Consumables (cups)
Glucometers
Glucose Tolerance Beverages
Hoods
HPLC Columns, Syringes,
andWater
Lipo Clear
Osmometers
PCR Plates
Pipettors
Reagents
Refrigerators
Serum Filters
SPE Columns
TDM Controls
TLC Plates
Tubes
Vials
Urinalysis
Hydrometers
Osmometers
Refractometers
Slides and Controls
Urinalysis Test Strips
Urine Centrifuge Tubes
Urine Sample Containers,
24Hrw/wo Preservatives
Urine Sediment Stains
Urine Transport Containers,
Sterile
VWRCatalystServices
Business Process Consulting
Chemical Tracking
Management
Equipment and Instruments
Services,AssetManagement
Equipment and Instruments
Services, Calibration
Equipment and Instruments
Services,Validation
Site Service Solutions
Immunology/Serology
Chemicals
Chromatography Filters
DNA/RNA Purification Products
DNA Collection Cards
Enzymes
Microarray Slides
Molecular Biology Reagents
PCR Plates
PCR Tubes, Caps, and Racks
Pipet Tips,Aerosol
Plate Readers andWashers
Rapid Tests
Sealing Film
Taq
Thermocyclers
Tissue CultureMedia
TissueGrinders
Lab Safety
Apparel
BiohazardWipes
Eyewear
Faceshields
Gloves
Gloves,Operating Sterile
Goggles
Handcare
Lab Coats
Matting,Anti-Fatigue
Monitors
Sharps Containers
Spill Control
WasteDisposalUnits,
Floor and Benchtop
Women’sHealth
BacterialVaginosis
Chlamydia
FSH
hCG (Serum/Urine)
hCGUrine Cassette
hCGUrine Strip
LH
Trichomonas
UCG
Furniture
Carts
Casework
Hoods
Pathology Stations
Safety Cabinets
Safety Showers and Eyewashes
Seating
Shelving
Water Systems
Workstations
Equipment& Instruments
Autoclaves
Balances
Centrifuges, Floor and Benchtop
Counters
Data Loggers
Decappers
Dispensers/Diluters
Freezers
FumeHoods
Incubators
Labelers
Microfuges
Microscopes
Osmometers
pHMeters
Pipettors, Single
andMultichannel
Plates, Stirring andHot
Printers
Refrigerators
Shakers
VortexMixers
Water Baths
Water Purification Systems
1.800.932.5000
|
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|
VWR International
|
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vii
|
VWR International
|
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|
1.800.932.5000
Application& ProductChecklist
Application& ProductChecklist
334
|
VWR International
|
vwr.com
|
1.800.932.5000
Molecular Diagnostics
PCR Kits
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